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Image Search Results
Journal: Cell chemical biology
Article Title: Hypomorph mutation-directed small-molecule protein-protein interaction inducers to restore mutant SMAD4-suppressed TGF-β signaling
doi: 10.1016/j.chembiol.2020.11.010
Figure Lengend Snippet: (A) Schematic illustration of the design of the TR-FRET assay for monitoring the SMAD4/SMAD3 PPI to discover small-molecule PPI inducers. Anti-Flag-Tb coupled with Flag-SMAD3 serves as the TR-FRET donor and anti-His-D2 coupled with His-SMAD4R361H serves as the acceptor. At the basal level, R361H impairs SMAD4 interaction with SMAD3, yielding low TR-FRET signal. Upon treatment with a PPI inducer, the induced SMAD3/SMAD4R361H complex formation brings two fluorophores into close proximity (<10 nm), generating a high TR-FRET signal.
Article Snippet: REAGENT or
Techniques:
Journal: Cell chemical biology
Article Title: Hypomorph mutation-directed small-molecule protein-protein interaction inducers to restore mutant SMAD4-suppressed TGF-β signaling
doi: 10.1016/j.chembiol.2020.11.010
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or
Techniques: Recombinant, Plasmid Preparation, Luciferase, Viability Assay, Software